What does PRP consist of?
Eye Platelet Rich Plasma (E-PRP) is a serum that contains an abundance of platelets, growth factors, white blood cells, mesenchymal stem cells, bioactive proteins, cytokines, and other potent elements. When used as eye drops, E-PRP provides the eye with the necessary building blocks to repair and regenerate tissues. This is possible because the serum is sourced from the patient’s own blood, making it safe to use.
To prepare the E-PRP serum, doctors follow a rigorous and meticulous process. They take a 50 ml blood sample from the patient’s forearm and add it to vacutainer tubes containing a citrate-dextrose solution. The tubes are then centrifuged at 200g for 11 minutes to separate the blood into three layers. The upper layers containing platelets and buffy coat are isolated and diluted with sterile saline solution. The resultant serum is divided into 5 ml bottles, which are wrapped in aluminum foil to protect the vitamin A content.
Patients are instructed to store the bottles at -20℃ until they are ready to use them. When using the serum, patients are advised to apply 1 drop in each eye along with preservative-free artificial tears every 2 hours during the day, along with a hyperosmotic agent every 4 hours. Doctors may also recommend therapeutic contact lenses to protect the cornea. The dosage is gradually reduced over a period of 4 months until patients use only PRP eye drops and preservative-free artificial tears. Patients are monitored through regular checkups at different intervals.